Improving the production of AHL lactonase AiiO-AIO6 from Ochrobactrum sp. M231 in intracellular protease-deficient Bacillus subtilis

Approaches for Improving Protein Production in Multiple Protease-Deficient Bacillus subtilis Host Strains

Cloning and Enhanced Expression of an Extracellular Alkaline Protease from a Soil Isolate of Bacillus clausii in Bacillus subtilis

in the detergent industry. In this study, the extracellular alkaline serine protease gene, aprE, from Bacillusclausii was amplified by PCR and further cloned and expressed in B. subtilis WB600 using the pWB980 expression vector. Protease activity of the recombinant B. subtilis WB600 harboring the plasmid pWB980/aprEreached up to 1020 U/ml, approximately 3-folds higher than the nativ...

Studies on Alkaline Protease Production from Bacillus sp

The aim of this study was optimization of the conditions for the production of alkaline protease from the Bacillus strains coded as L18, L21 and I18, which were isolated from our natural habitats. Additionally, the goal was also to optimize the production of alkaline protease from Bacillus sp. L21 in a new low-cost media formulation by employing design of experiments and response surface method...

Production and purification of a protease from an alkalophilic Bacillus sp. 2-5 strain isolated from soil

This research has focused on isolation and characterization of a strain of Bacillus <span style="font-variant: normal; font-style: normal; font-family: ArialMT...

Transient heterogeneity in extracellular protease production by Bacillus subtilis

The most sophisticated survival strategy Bacillus subtilis employs is the differentiation of a subpopulation of cells into highly resistant endospores. To examine the expression patterns of non-sporulating cells within heterogeneous populations, we used buoyant density centrifugation to separate vegetative cells from endospore-containing cells and compared the transcriptome profiles of both sub...